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      CRISPR & HiBiT Protein Tagging System at Universitätsklinikum Frankfurt, 27 May [LK01-0519] in Hochheim am Main


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      May 27, 2019

      Monday   2:30 PM

      Theodor-Stern-Kai 7 , Haus 22 Seminarraum 22-1
      Hochheim am Main, Hessen

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      CRISPR & HiBiT Protein Tagging System at Universitätsklinikum Frankfurt, 27 May [LK01-0519]

      This seminar offers an excellent opportunity to learn more about the CRISPR Technology and aboutthe newly developed bioluminescent HiBiT Protein Tagging System. Speakers:  Brian CaudillDirector of European SalesIntegrated DNA Technologies Dr. Erik BonkeApplication ScientistPromega Topics: Getting started with CRISPR: A review of gene knockout and homology-directedCRISPR has become an increasingly popular tool for genome editing, in part because it is highly flexible and relatively easy to implement compared to other technologies. However, for scientist beginning to work with this method, the wide range of products and variety of editing approaches can be overwhelming. In this seminar, Brian Caudill provides a simple explanation of the steps for planning your experiment, incluiding guide RNA design, an overview of delivery methods, and options for measuring editing results. He also discusses how to generate specific mutations in the genome using homology-directed repair (HDR). HiBiT Protein Tag - An ideal companion for CRISPR-mediated gene taggingThe investigation of protein regulatory processes in their natural environment is indispensable for scientists to understand biologic systems. This research is strongly advanced by CRISPR genomic enginieering that perfectly works hand in hand with the newly developed HiBiT Protein Tagging System. HiBiT is a bioluminescent peptide tag that can be easily appended to endogenous genes in a clonic-free CRISPR/Cas9 workflow. The striking innovation of HiBiT is reflected by its ability of quantify proteins even at low endogenous expression levels in an antibody-free fashion while providing a broad dynamic range. Here we present various applications of HiBiT system such as the setup of protein stabilization/degradation assay (i.e. transcription factors, PROTACS, ...) and GPCR internalization assays.

      Categories: Science

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