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Jan 9, 2009 (Friday)
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Event details: Can Individual DNA Bases Be Identified by Electron Tunnel...
Description
Seminar | January 9 | 2-3 p.m. | 3 LeConte Hall
Speaker/Performer: Prof. Stuart Lindsay, Arizona State Univ. Center for Single Molecule Biophysics
Sponsor: Berkeley Nanosciences and Nanoengineering Institute
Many schemes for very long reads of DNA sequence propose to pass the DNA through a nanopore so that each base appears at a reader in exactly the order in which it is incorporated into the parent polymer.
However, a reading device with single base resolution is required.
Electron tunneling has been proposed as a probe because the phenomenon is highly localized, and we have shown that the base composition of DNA oligomers can indeed be read by a chemically-functionalized tunneling probe. However, single base resolution cannot be obtained because of the size of the tunnel junction that is required to support a significant current.
Smaller single molecule targets are readily detected, and an alternative tunneling scheme will be described.
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